Blood transcriptomic signatures for symptomatic TB in an African multi-cohort study: Public, subject-level signature scores, RT-qPCR data, and metadata

Here we provide the public, subject-level datasets containing key variables necessary to reconstruct findings from the ScreenTB and AE-TBC sub-studies aimed at evaluating the diagnostic performance of multiple parsimonious transcriptomic signatures. A data dictionary is provided below.

Gene expression of 20 transcriptomic signatures was measured in study participants from study sites located in six different African countries where the ScreenTB and AE-TBC studies were conducted. Gene expression data was generated from microfluidic RT-qPCR runs on mRNA derived from whole blood collected in PAXgene tubes. All results were verified before delta Ct values were calculated and used to compute signature scores.

The public dataset for the sub-study is divided into (1) subject-level signature scores, (2) subject-level TaqMan RT-qPCR primer-probe assay raw cycle threshold (CT) gene expression data from the Standard BioTools microfluidic gene expression integrated fluidic circuits, and (3) subject-level metadata.