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Data used in the manuscript 'Mycobacterium tuberculosis-specific T cell functional, memory and activation profiles in QuantiFERON-reverters are consistent with controlled infection.'

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posted on 08.08.2021, 21:11 by Cheleka MpandeCheleka Mpande, Pia SteiglerPia Steigler, Tessa LloydTessa Lloyd, Virginie RozotVirginie Rozot, Boitumelo Mosito, Constance Schreuder, Timothy Reid, Nicole Bilek, Morten Ruhwald, Jason Andrews, Mark Hatherill, Francesca LittleFrancesca Little, Thomas ScribaThomas Scriba, Elisa NemesElisa Nemes

Reversion of immune sensitization tests for Mycobacterium tuberculosis (M.tb) infection, such as interferon-gamma release assays or tuberculin skin test, has been reported in multiple studies. We hypothesised that QuantiFERON-TB Gold (QFT) reversion is associated with a decline of M.tb-specific functional T cell responses, and a distinct pattern of T cell and innate responses compared to persistent QFT+ and QFT- individuals. This dataset consists of meta data from the Adolescent Cohort Study (ACS) and innate and adaptive cellular responses that were measured in stimulated Peripheral blood mononuclear cells (PBMCs).


PBMCs were collected at enrolment and at 6-monthly intervals during the 2-years of follow-up (termed months 0, 6, 12 and 18) when the QFT tests were performed to determine M.tb infection. Three groups of participants were defined based on their longitudinal QFT results: persistent QFT positives (QFT positive results > 0.35 IU/mL at four consecutive visits), QFT reverters (two QFT positive results, at least one > 0.7 IU/mL, followed by two QFT negative results, at least one < 0.2 IU/mL), and non-converters (QFT negative results < 0.2 at four consecutive visits). Overall, all three groups were matched by age, sex, ethnicity, school and known TB exposure.


Five stimulations were used to induce M.tb-specific T cell responses of the adaptive arm, including M.tb-specific peptide pools spanning ESAT-6/CFP-10 or EspC, EspF and Rv2348c (collectively termed Esp), and M.tb-lysate; Staphylococcus Enterotoxin B (SEB), as a positive control; or the cells were left unstimulated as a negative control. The variables include a combination of 5 effector functions (IL2, TNF, IFNg, CD107, CD154) produced by CD4+ and CD8+ T cells upon stimulation. Combinations of the phenotypic markers CD45RA, CCR7, CD27, KLRG1, HLA-DR and CXCR3 were further measured on IFNg, IL2 or TNF producing T cells (total Th1).


In the innate cells, effector responses were measured in unstimulated PBMC or after stimulation with M.tb-lysate or E. Coli (positive control). The variables in this dataset included a combination of 6 functional markers (Granzyme B, IL6, IL10, IL12, IFNg, TNF) produced by NK cells, B cells, monocytes, and DURT cells.


This dataset was used in the manuscript Mycobacterium tuberculosis-specific T cell functional, memory and activation profiles in QuantiFERON-reverters are consistent with controlled infection. See the full manuscript for more details.

Funding

US NIH (R21AI127121 and BAA-NIAID-NIHAI201700104)

Global Health Grant OPP1066265 from the Bill & Melinda Gates Foundation

Aeras and BMGF GC12 (grant 37885)

History

Department/Unit

South African Tuberculosis Vaccine Initiative