Data used in the manuscript 'Mycobacterium tuberculosis-specific T cell functional, memory and activation profiles in QuantiFERON-reverters are consistent with controlled infection.'

<p>Reversion of immune sensitization tests for <i>Mycobacterium tuberculosis</i> (<i>M.tb</i>) infection, such as interferon-gamma release assays or tuberculin skin test, has been reported in multiple studies. We hypothesised that QuantiFERON-TB Gold (QFT) reversion is associated with a decline of <i>M.tb</i>-specific functional T cell responses, and a distinct pattern of T cell and innate responses compared to persistent QFT+ and QFT- individuals. This dataset consists of meta data from the Adolescent Cohort Study (ACS) and innate and adaptive cellular responses that were measured in stimulated Peripheral blood mononuclear cells (PBMCs). </p><p><br></p><p>PBMCs were collected at enrolment and at 6-monthly intervals during the 2-years of follow-up (termed months 0, 6, 12 and 18) when the QFT tests were performed to determine <i>M.tb</i> infection. Three groups of participants were defined based on their longitudinal QFT results: persistent QFT positives (QFT positive results > 0.35 IU/mL at four consecutive visits), QFT reverters (two QFT positive results, at least one > 0.7 IU/mL, followed by two QFT negative results, at least one < 0.2 IU/mL), and non-converters (QFT negative results < 0.2 at four consecutive visits). Overall, all three groups were matched by age, sex, ethnicity, school and known TB exposure. </p><p><br></p><p>Five stimulations were used to induce <i>M.tb</i>-specific T cell responses of the adaptive arm, including <i>M.tb</i>-specific peptide pools spanning ESAT-6/CFP-10 or EspC, EspF and Rv2348c (collectively termed Esp), and<i> M.tb</i>-lysate; Staphylococcus Enterotoxin B (SEB), as a positive control; or the cells were left unstimulated as a negative control. The variables include a combination of 5 effector functions (IL2, TNF, IFNg, CD107, CD154) produced by CD4+ and CD8+ T cells upon stimulation. Combinations of the phenotypic markers CD45RA, CCR7, CD27, KLRG1, HLA-DR and CXCR3 were further measured on IFNg, IL2 or TNF producing T cells (total Th1).</p><p><br></p><p>In the innate cells, effector responses were measured in unstimulated PBMC or after stimulation with <i>M.tb</i>-lysate or <i>E. Coli </i>(positive control). The variables in this dataset included a combination of 6 functional markers (Granzyme B, IL6, IL10, IL12, IFNg, TNF) produced by NK cells, B cells, monocytes, and DURT cells.</p><p><br></p><p>This dataset was used in the manuscript <i>Mycobacterium tuberculosis-specific T cell functional, memory and activation profiles in QuantiFERON-reverters are consistent with controlled infection. </i>See the full manuscript for more details.</p><p></p>