DATASET
Combined results for the survival of freeze dried Lactobacillus in water, skimmed milk, inulin, maltodextrin and sucrose.xlsx (443.07 kB)
DATASET
Inhibiting activity of cryoprotectants.xlsx (580.76 kB)
DATASET
Survival of freeze dried L. plantarum in water, inulin and skimmed milk.xlsx (289.59 kB)
DATASET
Survival of freeze dried L. plantarum in water, maltodextrin and sucrose.xlsx (374.1 kB)
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SEM images of freeze dried L.plantarum in skimmed milk and water drying media_1.tif (952.69 kB)
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SEM images of freeze dried L.plantarum in skimmed milk and water drying media_2.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in skimmed milk and water drying media_3.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in skimmed milk and water drying media_4.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in skimmed milk and water drying media_5.tif (952.75 kB)
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SEM images of freeze dried L.plantarum in water (control drying media).tif (952.7 kB)
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SEM images of freeze dried L.plantarum in water (control drying media)_1.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in water (control drying media)_2.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in water (control drying media)_3.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in water (control drying media)_4.tif (952.71 kB)
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SEM images of freeze dried L.plantarum in inulin and water drying media_1.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in inulin and water drying media_2.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in inulin and water drying media_3.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in maltodextrin and water drying media_1.tif (952.71 kB)
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SEM images of freeze dried L.plantarum in maltodextrin and water drying media_2.tif (952.7 kB)
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SEM images of freeze dried L.plantarum in maltodextrin and water drying media_3.tif (952.71 kB)
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Impact of cryoprotectants during freeze drying on Lactobacillus plantarum viability and their role in enhancing probiotic storage stability
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posted on 2021-10-21, 07:59 authored by Sumbo OluwatosinSumbo Oluwatosin, Siew TaiSiew Tai, Marijke Fagan-EndresMarijke Fagan-EndresThis research investigated the prebiotic potential of various cryoprotectants and the protective effect of 10% (m/v) of skimmed milk, inulin, maltodextrin and sucrose as cryoprotectants on Lactobacillus plantarum during freeze drying. Improved survival of L. plantarum during freeze drying was observed with all cryoprotectants. Skimmed milk demonstrated the highest protection after freeze drying, with a survival rate of 91% and viable cell counts of 9.1 ×10^8 (CFU/ml) from an initial cell count prior to drying of 1.0×10^9 (CFU/ml). Inulin demonstrated high protective efficiency, with 85% viability maintained during freeze drying which resulted in final cell counts of 1.1×10^9 (CFU/ml) from an initial cell count of 1.3 ×10^9 (CFU/ml).
The data set contains:
1. OD and pH reading measured during the growth of Lactobacillus plantarum in media, each supplemented with various concentrations of glucose, inulin, maltodextrin and sucrose respectively
2. CFU counts and respective viabilities of L. plantarum after freeze drying and during storage periods, using various cryoprotectants.
3. OD and pH reading measured during the re- growth of Lactobacillus plantarum in media before freeze drying, after freeze during and over storage
4. SEM and TEM images of freeze dried L.plantarum in various cryoprotectants
