Plasmablastic lymphomas: EBV latency profile and MYC gene aberrations
datasetposted on 31.08.2021, 09:23 by Amsha RamburanAmsha Ramburan, Raymond Kriel, Dhirendra Govender
Plasmablastic lymphoma (PBL) is an aggressive disease that involves the Epstein-Barr virus (EBV), Human immunodeficiency virus (HIV) and MYC gene aberrations. We aimed to determine the EBV latent infection pattern and frequency of MYC gene aberrations in PBLs. Immunohistochemistry was performed using antibodies for EBNA1, EBNA2 and LMP1 while fluorescence in-situ hybridization was performed using a MYC probe. The patient cohort comprised 49 adult cases (44 were HIV-positive and 3 were HIV-negative). Forty-one cases were EBV-positive with 11 EBNA1-positive cases, all cases EBNA2-negative and 4 LMP1-positive cases. Latency 0 was determined in 29 cases, latency I in 8 cases and latency II in 4 cases. The MYC gene was rearranged in 8 cases, showed copy number variations in 11 cases and was intact in 11 cases. This is the largest cohort of PBLs from South Africa to show a predominantly restricted EBV latency pattern with MYC gene aberrations as a common finding.