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Secretion of cytokines, chemokines and antibodies in individuals with resistance to Mycobacterium tuberculosis infection

dataset
posted on 2023-03-28, 13:11 authored by Muki SheyMuki Shey, Avuyonke Balfour, Nomawethu Masina, Abulele Bekiswa, Charlotte SchutzCharlotte Schutz, Katalin WilkinsonKatalin Wilkinson, Rene Goliath, Rachel Dielle, Patrick D. M. C. Katoto, David M. Lewinsohn, Deborah A Lewinsohn, Graeme Meintjes

Cytokines and total antibodies in plasma and supernatants from Quantiferon-TB PLUS assay (Nil and TB1 stimulated) after inclubation for 16-20 hours. Concentrations were measured using luminex assays kits from Biorad (antibody isotyping panel) and Merck-Melliplex (high sensitivity T cell panel) according to manufacturers' instructions.


Samples were collected from individuals with latent TB infection (LTBI, IGRA+ & TST+), people with resistance to Mtb infection (Resisters, IGRA- & TST-) and people with discordant results for IGRA and TST (TST=0 and IGRA > 1IU/mL). IGRA+ for IFN-gamma level >0.35IU/mL; IGRA- for IFN-gamma <0.35IU/mL; TST+ for induration >10mm and TST- for induration <10mm

Funding

Wellcome Intermediate Fellowship Grant# 211360/Z/18/Z

National Research Foundation of South Africa, Grant# 127558

History

Department/Unit

Department of Medicine, University of Cape Town

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